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emem  (ATCC)


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    ATCC emem
    Emem, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6365 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eagles+minimum+essential+medium/Eagle's+Minimum+Essential+Medium/pm42844471-711-4-5
    Average 99 stars, based on 6365 article reviews
    emem - by Bioz Stars, 2026-10
    99/100 stars

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    Cell Culture:

    Article Title: Adipose-derived mesenchymal stromal cells alleviate intestinal fibrosis: The role of tumor necrosis factor-stimulated gene 6 protein.
    Article Snippet: Background: The therapeutic potential of adipose-derived mesenchymal stromal cells (AMSCs) in the treatment of intestinal fibrosis occured in patients with Crohn’s disease (CD) remains unclear.. Tumor necrosis factorstimulated gene 6 (TSG6) protein plays a critical role in inflammation regulation and tissue repair.. This study aimed to determine if AMSCs attenuate intestinal fibrosis by secreting paracrine TSG6 protein and explore the underlying mechanisms.

    Article Title: Design, Synthesis, and Biophysical Characterization of Pyridine Bis‐Quinazoline Derivatives as Selective G‐Quadruplex DNA Stabilizers
    Article Snippet: Two tumor cell‐lines, the colorectal HCT‐8 (kindly provided by Prof. Christian Hedberg at Umeå University) and the hepatocellular carcinoma cell‐line HepG2 (HB‐806TM, ATCC®), were used to measure cytotoxicity of the compounds. .. HCT‐8 were cultured in RPMI‐1640 (GIBCO) and HepG2 in Eagles Minimum Essential Medium (ATCC), both supplemented with 10 % FBS (GIBCO) and 100 U Penicillin/Streptomycin (GIBCO) at 37 °C, 5 % CO 2 . .. The cells were harvested at around 70 % confluency by 0.25 % trypsin EDTA (GIBCO) and seeded in 384 well microtiter plates (FalconTM OptiluxTM 384‐Well, Cell Culture‐Treated, Flat‐Bottom, fisher scientific) at a density of 2000/well in 50 μl culture media (CM).

    Article Title: Synthesis of small molecules inspired by phomoxanthone A
    Article Snippet: .. Jurkat and L5178Y cells were cultured in RPMI 1640 (ATCC), HL60 cells were cultured in Iscove's Modified Dulbecco's Medium (IMDM, ATCC), KB and MCF7 cells were cultured in Eagles Minimum Essential Medium (EMEM, ATCC), and SKOV3 cells in McCoy's 5a Medium (ATCC) supplemented with 10% Heat Inactivated Fetal Bovine Serum (FBS), and Penicillin and Streptomycin (P/S) at 37° C. in a 5% CO2 incubator. ..

    other:

    Article Title: MicroRNA-873 Suppresses Viability and Invasion of Colorectal Cancer Through KRAS/MAPK Signaling and Sensitizes Tumor Spheroids to 5-Fluorouracil in a 3D Microwell Model
    Article Snippet: RKO cells were maintained in Eagles Minimum Essential Medium (EMEM; ATCC) with 10 FBS and 1 penicillinstreptomycin.

    Electroporation:

    Article Title: Simulating a for-loop in the human genome: Design and evaluation of recombinase genetic programs that count to three
    Article Snippet: This mixture was transferred into one well of a 96-well Nucleocuvette plate (Lonza) and electroporated using protocol DS150. .. Following electroporation, cells were transferred to a 96-well plate preheated with Eagles Minimum Essential Medium (EMEM; ATCC: 30-2003). ..

    Modification:

    Article Title: Synthesis of small molecules inspired by phomoxanthone A
    Article Snippet: .. Jurkat and L5178Y cells were cultured in RPMI 1640 (ATCC), HL60 cells were cultured in Iscove's Modified Dulbecco's Medium (IMDM, ATCC), KB and MCF7 cells were cultured in Eagles Minimum Essential Medium (EMEM, ATCC), and SKOV3 cells in McCoy's 5a Medium (ATCC) supplemented with 10% Heat Inactivated Fetal Bovine Serum (FBS), and Penicillin and Streptomycin (P/S) at 37° C. in a 5% CO2 incubator. ..



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    Average 99 stars, based on 1 article reviews
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    LINC00887 is highly expressed and its impact on aerobic glycolysis in ccRCC, (A) Analysis of LINC00887 mRNA levels in ccRCC and adjacent normal tissues from TCGA-KIRC dataset. (B) qRT-PCR test of LINC00887 mRNA levels in HK-2, 786-O, Caki-1, and A498 cell lines. (C) KEGG pathway analysis of signaling pathways enriched by LINC00887 in ccRCC. (D) KEGG analysis showing enrichment of LINC00887 in the glycolysis pathway in ccRCC. (E) Pearson correlation analysis between LINC00887 and glycolytic marker genes. 786-O cells were transfected with either oe-NC or oe-LINC00887, respectively. (F) qRT-PCR was performed to verify transfection efficiency. (G) qRT-PCR measurement of glycolytic marker genes LDH-A, HK2, and GLUT1 mRNA levels in each group. (H) immunoblotting analysis of glycolytic marker LDH-A, HK2, and GLUT1 protein levels in each group. (I) Seahorse metabolic analyzer was used to measure ECAR in each group, calculating glycolytic value and glycolytic capacity. (J) Seahorse metabolic analyzer was used to measure OCR in each group, calculating ATP production, and maximal respiration. (K–M) Kits were used to measure glucose consumption (K) , lactate production (L) , and ATP yield (M) . *indicates p < 0.05. ccRCC, Clear cell renal cell carcinoma; qRT-PCR, Quantitative reverse transcription polymerase chain reaction; KEGG, Kyoto Encyclopedia of Genes and Genomes; LDH-A, Lactate dehydrogenase A; HK2, Hexokinase 2; GLUT1, Glucose transporter 1; ECAR, Extracellular acidification rate; OCR, Oxygen consumption rate; ATP, Adenosine triphosphate.

    Journal: Frontiers in Immunology

    Article Title: UPF1-dependent glycolysis driven by LINC00887 facilitates macrophage M2 polarization in clear cell renal cell carcinoma

    doi: 10.3389/fimmu.2026.1833383

    Figure Lengend Snippet: LINC00887 is highly expressed and its impact on aerobic glycolysis in ccRCC, (A) Analysis of LINC00887 mRNA levels in ccRCC and adjacent normal tissues from TCGA-KIRC dataset. (B) qRT-PCR test of LINC00887 mRNA levels in HK-2, 786-O, Caki-1, and A498 cell lines. (C) KEGG pathway analysis of signaling pathways enriched by LINC00887 in ccRCC. (D) KEGG analysis showing enrichment of LINC00887 in the glycolysis pathway in ccRCC. (E) Pearson correlation analysis between LINC00887 and glycolytic marker genes. 786-O cells were transfected with either oe-NC or oe-LINC00887, respectively. (F) qRT-PCR was performed to verify transfection efficiency. (G) qRT-PCR measurement of glycolytic marker genes LDH-A, HK2, and GLUT1 mRNA levels in each group. (H) immunoblotting analysis of glycolytic marker LDH-A, HK2, and GLUT1 protein levels in each group. (I) Seahorse metabolic analyzer was used to measure ECAR in each group, calculating glycolytic value and glycolytic capacity. (J) Seahorse metabolic analyzer was used to measure OCR in each group, calculating ATP production, and maximal respiration. (K–M) Kits were used to measure glucose consumption (K) , lactate production (L) , and ATP yield (M) . *indicates p < 0.05. ccRCC, Clear cell renal cell carcinoma; qRT-PCR, Quantitative reverse transcription polymerase chain reaction; KEGG, Kyoto Encyclopedia of Genes and Genomes; LDH-A, Lactate dehydrogenase A; HK2, Hexokinase 2; GLUT1, Glucose transporter 1; ECAR, Extracellular acidification rate; OCR, Oxygen consumption rate; ATP, Adenosine triphosphate.

    Article Snippet: Caki-1 cells were kept in 5A medium (30-2007, ATCC, USA), 786-O cells in DMEM-H (11965092, Gibco, USA), and A498 cells in EMEM (30-2003, ATCC, USA).

    Techniques: Analysis, Quantitative RT-PCR, Protein-Protein interactions, Marker, Transfection, Western Blot, Reverse Transcription, Polymerase Chain Reaction

    LINC00887-mediated UPF1 suppresses ccRCC malignant progression, (A) mRNA levels of UPF1 in ccRCC tumor tissues versus adjacent normal tissues as analyzed by TCGA-KIRC dataset. (B) qRT-PCR detection of UPF1 mRNA in HK-2, Caki-1, 786-O, and A498 cells. (C) Immunoblotting assessment of UPF1 protein in renal cell lines. (D) CCK-8 assay for cell viability in 786-O cells expressing sh-NC or sh-UPF1 and Caki-1 cells expressing oe-NC or oe-UPF1. (E) Colony formation assay determined cell proliferation in the transfected cells. (F) Scratch assay evaluated cell migration. (G) Transwell assessed cell invasion. (H) Flow cytometry analyzed apoptosis in different cell groups. (I) Flow cytometry examined cell cycle distribution in various cell groups. *, p < 0.05. ccRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1. qRT-PCR, Quantitative reverse transcription polymerase chain reaction; TCGA, The cancer genome atlas; KIRC, Kidney renal clear cell carcinoma.

    Journal: Frontiers in Immunology

    Article Title: UPF1-dependent glycolysis driven by LINC00887 facilitates macrophage M2 polarization in clear cell renal cell carcinoma

    doi: 10.3389/fimmu.2026.1833383

    Figure Lengend Snippet: LINC00887-mediated UPF1 suppresses ccRCC malignant progression, (A) mRNA levels of UPF1 in ccRCC tumor tissues versus adjacent normal tissues as analyzed by TCGA-KIRC dataset. (B) qRT-PCR detection of UPF1 mRNA in HK-2, Caki-1, 786-O, and A498 cells. (C) Immunoblotting assessment of UPF1 protein in renal cell lines. (D) CCK-8 assay for cell viability in 786-O cells expressing sh-NC or sh-UPF1 and Caki-1 cells expressing oe-NC or oe-UPF1. (E) Colony formation assay determined cell proliferation in the transfected cells. (F) Scratch assay evaluated cell migration. (G) Transwell assessed cell invasion. (H) Flow cytometry analyzed apoptosis in different cell groups. (I) Flow cytometry examined cell cycle distribution in various cell groups. *, p < 0.05. ccRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1. qRT-PCR, Quantitative reverse transcription polymerase chain reaction; TCGA, The cancer genome atlas; KIRC, Kidney renal clear cell carcinoma.

    Article Snippet: Caki-1 cells were kept in 5A medium (30-2007, ATCC, USA), 786-O cells in DMEM-H (11965092, Gibco, USA), and A498 cells in EMEM (30-2003, ATCC, USA).

    Techniques: Quantitative RT-PCR, Western Blot, CCK-8 Assay, Expressing, Colony Assay, Transfection, Scratch Assay, Migration, Flow Cytometry, Reverse Transcription, Polymerase Chain Reaction